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genepulser xcell microbial system  (Bio-Rad)


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    Bio-Rad genepulser xcell microbial system
    Genepulser Xcell Microbial System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 106 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/genepulser+xcell+microbial+system/Gene+Pulser+Xcell+Microbial+System/pm41436063-158-25-24
    Average 94 stars, based on 106 article reviews
    genepulser xcell microbial system - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Transformation Assay:

    Article Title: Recoded Bacteriophage Genome for Bio-Orthogonal-Enabled Concentration and Detection of E. coli in Drinking Water
    Article Snippet: .. A portion of the assembly reactions (1 μL) were transformed into E. coli NEB 10β (C3020, NEB) cells by electroporation utilizing a BioRad GenePulser Xcell Microbial System under standard conditions (1.8 kV, 25 μF, 200 Ω). .. Following electroporation, cells were recovered in NEB 10-beta/Stable Outgrowth Medium (B9035, NEB) at 37 °C with agitation for 1.5 h. 10–500 μL of recovery culture were mixed with 3 mL of 0.7% molten LB Lennox top agar and plated onto LB Lennox plates.

    Article Title: Recoded Bacteriophage Genome for Bio-Orthogonal-Enabled Concentration and Detection of E. coli in Drinking Water.
    Article Snippet: .. Phage Rescue and Sequencing.A portion of the assembly reactions (1 μL) were transformed into E. coliNEB 10β (C3020, NEB) cells by electroporation utilizing a BioRad GenePulser Xcell Microbial System under standard conditions (1.8 kV, 25 μF, 200 Ω). .. Following electroporation, cells were recovered in NEB 10-beta/Stable Outgrowth Medium (B9035, NEB) at 37 °C with agitation for 1.5 h. 10−500 μL of recovery culture were mixed with 3 mL of 0.7% molten LB Lennox top agar and plated onto LB Lennox plates.

    Electroporation:

    Article Title: Recoded Bacteriophage Genome for Bio-Orthogonal-Enabled Concentration and Detection of E. coli in Drinking Water
    Article Snippet: .. A portion of the assembly reactions (1 μL) were transformed into E. coli NEB 10β (C3020, NEB) cells by electroporation utilizing a BioRad GenePulser Xcell Microbial System under standard conditions (1.8 kV, 25 μF, 200 Ω). .. Following electroporation, cells were recovered in NEB 10-beta/Stable Outgrowth Medium (B9035, NEB) at 37 °C with agitation for 1.5 h. 10–500 μL of recovery culture were mixed with 3 mL of 0.7% molten LB Lennox top agar and plated onto LB Lennox plates.

    Article Title: Recoded Bacteriophage Genome for Bio-Orthogonal-Enabled Concentration and Detection of E. coli in Drinking Water.
    Article Snippet: .. Phage Rescue and Sequencing.A portion of the assembly reactions (1 μL) were transformed into E. coliNEB 10β (C3020, NEB) cells by electroporation utilizing a BioRad GenePulser Xcell Microbial System under standard conditions (1.8 kV, 25 μF, 200 Ω). .. Following electroporation, cells were recovered in NEB 10-beta/Stable Outgrowth Medium (B9035, NEB) at 37 °C with agitation for 1.5 h. 10−500 μL of recovery culture were mixed with 3 mL of 0.7% molten LB Lennox top agar and plated onto LB Lennox plates.

    Pulsed-Field Gel:

    Article Title: An in vitro CRISPR-Cas12a-mediated protocol for direct cloning of large DNA fragments.
    Article Snippet: Note: 150 mL cleavage mix is only enough for one plug, the volume of mix can be adjusted according to the number of plugs. b. Incubate the tube at 37 C for 1 h. c. Discard liquid and rinse the plug in the tube with 1 mL DNase/RNase-Free Distilled Water (ThermoFisher, Catalog# A57775). .. Optional: Cut 1/4 plug using a clean blade and run the pulsed field gel electrophoresis (PFGE) with GenePulser Xcell microbial system (Bio-Rad, Catalog# 1652662) to make sure that the target DNA fragment had been cleaved (Figure 4B). .. MidRange PFG Marker (NEB, Catalog# N0342s) is used as control.

    Electrophoresis:

    Article Title: An in vitro CRISPR-Cas12a-mediated protocol for direct cloning of large DNA fragments.
    Article Snippet: Note: 150 mL cleavage mix is only enough for one plug, the volume of mix can be adjusted according to the number of plugs. b. Incubate the tube at 37 C for 1 h. c. Discard liquid and rinse the plug in the tube with 1 mL DNase/RNase-Free Distilled Water (ThermoFisher, Catalog# A57775). .. Optional: Cut 1/4 plug using a clean blade and run the pulsed field gel electrophoresis (PFGE) with GenePulser Xcell microbial system (Bio-Rad, Catalog# 1652662) to make sure that the target DNA fragment had been cleaved (Figure 4B). .. MidRange PFG Marker (NEB, Catalog# N0342s) is used as control.

    Sequencing:

    Article Title: Recoded Bacteriophage Genome for Bio-Orthogonal-Enabled Concentration and Detection of E. coli in Drinking Water.
    Article Snippet: .. Phage Rescue and Sequencing.A portion of the assembly reactions (1 μL) were transformed into E. coliNEB 10β (C3020, NEB) cells by electroporation utilizing a BioRad GenePulser Xcell Microbial System under standard conditions (1.8 kV, 25 μF, 200 Ω). .. Following electroporation, cells were recovered in NEB 10-beta/Stable Outgrowth Medium (B9035, NEB) at 37 °C with agitation for 1.5 h. 10−500 μL of recovery culture were mixed with 3 mL of 0.7% molten LB Lennox top agar and plated onto LB Lennox plates.



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    Image Search Results


    Journal: STAR Protocols

    Article Title: An in vitro CRISPR-Cas12a-mediated protocol for direct cloning of large DNA fragments

    doi: 10.1016/j.xpro.2023.102435

    Figure Lengend Snippet:

    Article Snippet: Optional: Cut 1/4 plug using a clean blade and run the pulsed field gel electrophoresis (PFGE) with GenePulser XcellTM microbial system (Bio-Rad, Catalog# 1652662) to make sure that the target DNA fragment had been cleaved ( B).

    Techniques: Virus, Recombinant, Marker, Cloning, Plasmid Preparation, Modification, Software, CRISPR, Spectrophotometry, Imaging, Electrophoresis